Structural analysis of κ-carrageenan sulfated oligosaccharides by positive mode Nano-ESI-FTICR-MS and MS/MS by SORI-CID

Structural Analysis of -Carrageenan Sulfated Oligosaccharides by Positive Mode Nano-ESI- FTICR-MS and MS/MS by SORI-CID

Structural analysis of sulfated oligosaccharides from -carrageenan of up to ten residues (MW 2 kDa) was successfully carried out by positive mode nano-ESI-FTICR-MS together with MS/MS using sustained off-resonance irradiation-collision induced dissociation (SORI-CID). Glycosidic bond cleavage reactions via the Band Y-types of fragmentation were observed and enabled complete sequencing of the ol...

ESI and nanoESI - CID - MS / MS

Structural characterisation by ESI-MS of feruloylated arabino-oligosaccharides synthesised by chemoenzymatic esterification.

The chemoenzymatic synthesis of feruloylated arabino-oligosaccharides has been achieved, using a feruloyl esterase type C from Sporotrichum thermophile (StFaeC). The structure of the feruloylated products was confirmed by ESI-MS(n).

Quantification of Signaling Lipids by Nano-Electrospray Ionization Tandem Mass Spectrometry (Nano-ESI MS/MS)

Lipids, such as phosphoinositides (PIPs) and diacylglycerol (DAG), are important signaling intermediates involved in cellular processes such as T cell receptor (TCR)-mediated signal transduction. Here we report identification and quantification of PIP, PIP2 and DAG from crude lipid extracts. Capitalizing on the different extraction properties of PIPs and DAGs allowed us to efficiently recover b...

Structural Characterization of Sulfoquinovosyl, Monogalactosyl and Digalactosyl Diacylglycerols by FAB-CID-MS/MS

The glycolipids such as sulfoquinovosyl diacylglycerol (SQDG), isolated from wild-type cyanobacterium Synechocystis sp. PCC 6803, and monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG), obtained from whole wheat Ñour, have been investigated by using fast atom bombardment tandem mass spectrometry (FAB-MS/MS). Collision-induced dissociation (CID) of sodium-adduct molecula...